Metadata (abstracts and keywords) for the articles in the journal
S. A. Koshkin, M. A. Ivanova, G. V. Timin, I. V. Rykov, I. A. Chistyakov, E. N. Tolkunova INCREASED DRUG RESISTANCE IN CANCER STEM CELLS UNDER THE CONDITIONS OF HYPOXIA // I. YAKOVLEV CHUVASH STATE PEDAGOGICAL UNIVERSITY BULLETIN. 2016. № 4(92). p. 23-33
Author(s):
S. A. Koshkin, M. A. Ivanova, G. V. Timin, I. V. Rykov, I. A. Chistyakov, E. N. Tolkunova
Index of UDK:
616.34-006.6-039.73
Index of DOI:
Name of article:
INCREASED DRUG RESISTANCE IN CANCER STEM CELLS UNDER THE CONDITIONS OF HYPOXIA
Keywords:
colon cancer, cancer stem cells, CoCl2, hypoxia, drug resistance.
Abstracts:
Overcoming the problems of drug resistance and metastasis of separate malignant tumors remains one of the most pressing problems of oncology. According to the modern concepts of carcinogenesis, the development of tumor process is caused by the operation of a small population of cancer stem cells (CSCs) resistant to external influences. One of the factors that inherently accompany the growth and development of tumors are tissue and cellular hypoxia. The study presented in this article was aimed at studying the resistance of CSC to chemotherapy under hypoxic conditions. Cancer stem cells have been isolated by two viral transduction of primary colon cancer lines obtained from surgical specimens. For the selection of cells, that are expressing markers, stem SORE6x reporter construct were used. The expression of stem markers was evaluated by RT-PCR. The isolated cells were cultured under hypoxic conditions simulated with CoCl2 introduction into the culture medium. As the cytostatic agent is widely used in the treatment of colon cancer drug – 5-Fluorouracil. Evaluation of the number of cells and their viability was made using MTT method. It is shown that the use of this reporter construct allows to obtain stem cells expressing markers. These cells are characterized by increased resistance to 5-FU. Impact of hypoxia on their substantially increases resistance.
